Candidate probiotic bacteria were searched for use as probiotic against vibriosis in rainbow trout in this study. A total of 143 bacterial strains were isolated from rainbow trout rearing wa-ter, gills and skin mucus and tested for antagonistic activity against V. anguillarum. Antagonis-tic strains were characterized for enzymatic activity (protease, lipase) and hydrophobicity. Bac-teria belonging to the genus Aeromonas and Pseudomonas showed inhibitory activity against V. anguillarum. Aeromonas sp. HS23 showed protease and lipase activity, while Aeromonas sp. MS30 displayed only lipase activity. Pseudomonas spp. did not display enzymatic activity. On the other hand, all bacteria showed hydrophobicity indicating their adherence capability to the host. However, these strains should be further studied to explore their probiotic effects in vivo.